Fig. 4.

Chromosomal rec10 NLS mutations block entry of Rec25–GFP, Rec27–GFP and Mug20–GFP into the nucleus. (A) Strains bearing rec25–GFP and without (WT) or with the indicated rec10 NLS mutations were harvested during asynchronous (h⁹⁰) meiosis and examined by light microscopy. (B) Strains bearing rec27–GFP were analyzed as in panel A. (C) Strains bearing mug20–GFP were analyzed as in panel A. In the merged images, green indicates GFP, and red indicates chromatin in the nucleus; yellow is their overlap. See Fig. 2 for examples of separate images for Rec25–GFP and chromatin. The dashed line outlines the cell. NLS mutations are designated as in Fig. 2. Images are representative of >50 cells from three separate cultures analyzed on different days. Cells are in the horsetail stage, as in Fig. 2. Scale bars: 5 μm.