Table 3.

Primer sequences, manufacturer’s, product sizes (bp), concentrations (conc.) and programme used for qRT-PCR measurement

Primer	Sequence	Size [bp]	conc [µM]	Annealing Temp (time) ( no. of cycles)
PGK1	5′-GCAGATTGTTTGGAATGGTC-3’ 5′-TGCTCACATGGCTGACTTTA-3’	185	0.4	58 °C (45 s), (45x)
B2M	5′-GGCCTGTATGCTATCCAGAA-3’ 5′-GAAAGACCAGTCCTTGCGA-3’	198	0.4	58 °C (45 s), (45x)
BDNF	5′-GATGCCAGTTGCTTTGTCTT-3’ 5′-ATGTGAGAAGTTCGGCTTTG-3’	137	0.1	58 °C (45 s), (42x)
NGF	5′-ATCAAGGGCAAGGAGGTGACAG- ‘3 5′-GAGTTCCAGTGTTTGGAGTCGATG-3 ‘	143	0.15	62 °C (20 s), (40x)
NRF2	5′-GATCTCCTCGCTGGAAAAAG-3’ 5′-GTCACTGGGCTCTGCTATGA-3’	187	0.4	56 °C (30 s), (50x)
CREB1	5′-TAGCTGTGACTTGGCATTCA-3’ 5′-TTGTTCTGTTTGGGACCTGT-3’	184	0.5	58 °C (45 s), (45x)
Synaptophysin	5′-TTTGTGGTTGTTGAGTTCCT-3’ 5′-GCATTTCCTCCCCAAAGTAT-3’	204	0.1	58 °C (45 s), (42x)
Gap43	5′-AGGGAGATGGCTCTGCTACT-3’ 5′-GAGGACGGGGAGTTATCAGT-3’	190	0.15	58 °C (45 s), (42x)
Keap1	5′-ATGGCCACACTTTTCTGGAC-3’ 5′-TCCTGTTGTCAGTGCTCAGG-3’	131	0.2	60 °C (45 s), (45x)
IL1β	5′-CCCAACTGGTACATCAGCAC-3’ 5′-TCTGCTCATTCACGAAAAGG-3’	180	0.3	58 °C (30 s), (45x)