FIGURE 5.

Pollen grain size and germination rate of the rdr6-12/FH13-YFP OX transgenic overexpression line (A) Representative confocal maximum intensity fluorescence projection (left) and single bright field optical section (right) of pollen from a rdr6-12 plant heterozygous for the FH13-YFP OX transgene after 30 min and 4 h of culture. Note the difference between the fluorescent (transgenic) and non-fluorescent (non-transgenic) pollen grains. (B) Comparison of pollen grain area of non-transgenic (no signal) and rdr6-12/FH13-YFP OX (fluorescent) pollen grains at 30 min after plating (n > 300); NS, non-significant difference (one-way ANOVA, Tukey test p > 0.05). (C) Fraction of germinated pollen grains after the indicated time of in vitro cultivation. For genotype description see (B); error bars represent ± SD from three technical replicates. Difference between transgenic and non-transgenic pollens at each of the time points were non-significant (χ² test, p > 0.05).