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. 2021 Feb 18;12:599961. doi: 10.3389/fpls.2021.599961

FIGURE 6.

FIGURE 6

In vitro pollen tube growth of transgenic lines. (A) Representative single optical sections of pollen tubes from an rdr6-12 plant heterozygous for the FH13-YFP OX transgene, taken at a 30 min interval 4 h after sowing on solid medium. Fluorescence (top) and bright field (bottom) channels are shown in grayscale. Note the presence of both fluorescent (i.e., transgenic) and non-fluorescent (non-transgenic) meiotic segregants in the same field. (B) Growth rates of non-transgenic (no signal) and FH13-YFP OX rdr6-12 (fluorescent) pollen tubes (n > 40) from the line shown in (A). Asterisks indicate a statistically significant difference (one-way ANOVA, Tukey test, ** for p < 0.01). (C) Representative single optical sections of pollen tubes from transgenic plants derived either from WT (top) or fh13-1 (bottom) background and heterozygous for the FH13-Venus transgene, taken at a 30 min interval 4 h after sowing on solid medium. Fluorescence (top) and bright field (bottom) channels are shown in grayscale, presenting both transgenic and non-transgenic meiotic segregants. (D) Growth rates of non-transgenic (no signal) and FH13-Venus (fluorescent) pollen tubes from plant lines with either WT of fh13-1 genetic background (n > 20). (E) Fluorescence intensities of FH13-Venus in WT and fh13-1 mutant backgrounds (signal) and controls (no signal) measured from single optical sections of pollen grains (in arbitrary units, n > 30). Significant differences in (D,E) are marked by different letters (one-way ANOVA, Tukey test, p < 0.01).