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. 2021 Mar 3;11:5088. doi: 10.1038/s41598-021-84427-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Piezo1 plaques correlate with FAs at the cell extrusion edges on the pattern. (a) Images of a typical HEK-hP1 cell (Piezo1, green) co-expressing mApple-paxillin (red); the zoomed view shows the co-localization between Piezo1 plaques and FAs at the extrusion edge at various times during cell elongation. (b) Statistical data shows that the two proteins collocated in most elongated cells, but side by side in some cells (n = 62 for patterned cells, n = 25 for control cells). (c) Images of paxillin and Piezo1 in a control cell (without pattern) show that there are no mature FAs present at the cell edges. Scale bars represent 10 µm.