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. 2021 Mar 3;12:1413. doi: 10.1038/s41467-021-21666-7

Fig. 2. Photochromic behavior of RFPs in U-2 OS cells.

Fig. 2

The cells were widefield illuminated with alternating light of 561 nm and 488 nm (a–c) or 405 nm (e–g) lights for multiple illumination cycles. The photochromic amplitudes (blue arrows) of RFPs determined for each cycle are presented in (d) and (h). Boxplots were generated using BoxPlotR (http://boxplot.tyerslab.com/), which includes the median values, the individual data points, and the 95% confidence intervals (notches). d n = 14 cells for pHmScarlet, n = 14 cells for mScarlet, and n = 19 cells for pHuji examined. Independent experiments were repeated twice for each FPs. The median, upper whisker, and lower whisker are 5%, 17%, and –9% for pHmScarlet, 3%, 15%, and –13% for mScarlet, and 34%, 55%, and 18% for pHuji, respectively. h n = 5 cells for pHmScarlet, n = 7 cells for mScarlet, and n = 4 cells for pHuji. Independent experiments were repeated three times for each FPs. The median, upper whisker, and lower whisker are 1%, 5%, and –4% for pHmScarlet, 3%, 10%, and –10% for mScarlet, and 35%, 52%, and 16% for pHuji, respectively. a, e: pHmScarlet; b, f: mScarlet; c, g: pHuji. Source data is provided as a source data file.