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Number of annotated metabolites in the intracellular and extracellular medium after incubation of HK-2 cells in HG (25 mM glucose)-hypoxia (1% O2) and control (5.5 mM glucose/18.6% O2) conditions for 48 h and using different analytical platforms and ionization modes.
| Platform | Intracellular medium | Extracellular medium |
|---|---|---|
| GC-TOF MS | 142 | 81 |
| HILIC-QE MS/MS ( +) | 246 | 168 |
| HILIC-QE MS/MS ( −) | 141 | 92 |
| CSH-QE MS/MS ( +) | 194 | 11 |
| CSH-QE MS/MS ( −) | 280 | 16 |
| Total | 733 | 290 |
