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. 2021 Mar 3;12(3):228. doi: 10.1038/s41419-021-03536-8

Fig. 2. The effect of WBP2 on lipid deposition in primary hepatocytes stimulated by PA.

Fig. 2

A, C A WBP2-overexpressing cell line (A) and a WBP2 knockout cell line (C) were established in LO2 cells. The protein expression of WBP2 was tested by western blot analysis. B Representative photomicrographs with Nile red staining are shown for the control and WBP2-overexpressing cells exposed to PA (0.25 mM) for 12 h. D Representative photomicrographs with Nile red staining are shown for the WT and WBP2 KO cells exposed to PA (0.25 mM) for 12 h. The Nile red-stained area was quantified by ImageJ software. Scale bar, 100 μm. Data are expressed as the mean ± SEM, ***P < 0.001.