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. 2021 Mar 3;11:5085. doi: 10.1038/s41598-021-84106-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of LOTUS decreases the density of PSD95/Bassoon puncta in cultured hippocampal neurons. (a, b) Cultured hippocampal neurons (DIV 14) derived from WT (a) and LOTUS-KO (b) mice. Neurons were immunostained with antibodies against Bassoon (red), PSD-95 (green), and MAP2 (blue). Scale bars, 10 µm. (c, d) Magnified images from (a) and (b). The segment was imaged at 3 × magnification. Scale bars, 10 µm. (e) Quantification of the synaptic density of Bassoon/PSD95 puncta along the dendrites of each neuron. Data were normalized to the synaptic density in WT neurons. Data are means ± SEM from four to five independent experiments. The total number of neurons analyzed (n) ranged from 16 to 20 cells per condition. ***P < 0.001, Student’s unpaired t-test.