Figure 5.

Loss of LOTUS causes impairment in spatial learning. (a) Time course of the experimental procedure of the Morris Water Maze test. (b) Escape latency time in WT and LOTUS-KO mice. Data are means ± SEM from WT mice (n = 15) and LOTUS-KO mice (n = 13). *P < 0.05, Student’s unpaired t-test. (c) Test in WT and LOTUS-KO mice after training for 3 days. Data are analyzed by χ² test. (d) Test in WT and LOTUS-KO mice after training for 6 days. Data are means ± SEM from WT (n = 15) and LOTUS-KO (n = 13) mice. **P < 0.01, one-way ANOVA with post-hoc Steel − Dwass test and with χ² test. Opposite (OP); adjacent right (AR); target quadrant (TQ); adjacent left (AL). (e) Ratio of time spent in the TQ Ward during the test. Data are means ± SEM from WT (n = 15) and LOTUS-KO (n = 13) mice. ***P < 0.001, Student’s unpaired t-test.