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. 2021 Mar 3;11:5098. doi: 10.1038/s41598-021-84513-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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H3.3K27M mutant DNA is detectable in patient-matched tumor tissue, CSF and plasma or serum specimens. Patient-matched specimens from children with H3.3K27M mutant DMG (n = 3) were analyzed on the BioRad and RainDance platforms using Assay A. Mutant droplets were detected in all specimens across each subject tested, with greater MAF observed on the BioRad platform (t-test). Due to higher extracted [ctDNA] from DMG-128 CSF and plasma, samples were diluted 1:10 in DNA suspension buffer to prevent probe signal oversaturation. All other samples were diluted 1:5 as described. % values = Mutation Allelic Frequency (MAF); * = Statistically significant difference in MAF (t-test, p < 0.05).