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. 2021 Mar 3;11:5098. doi: 10.1038/s41598-021-84513-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ddPCR results are consistent across institutions, irrespective of specimen preparation location. To determine the effects of location of sample DNA extraction and specimen shipment on ddPCR analysis results, DNA was extracted from (A) H3K27M mutant DMG CSF (DMG-1-C), and (B) DMG cells (SF 7761) at NU. ctDNA was pre-amplified using Assay C primers (NU), then analyzed on BioRad (NU) and RainDance (CN) platforms, with results compared to those from ctDNA extracted at CN. As with CN-prepared specimens, NU-prepared specimens yielded greater relative positive droplet counts in cell-derived ctDNA compared to CSF, and on RainDance compared to BioRad, with consistent droplet counts and MAFs between assays within a given platform. % values = Mutation Allelic Frequency (MAF).