Table 1.

Custom, sequence specific primers and fluorescent locked nucleic acid (LNA) probe sets utilized.

	Assay A (Pandarithna et al., CN)	Assay B (Stallard et al., UM)	Assay C (Huang et al., NU)
Forward primer	5′-GTACAAAGCAGACTGCCCGCAAAT-3′	5′-GGTAAAGCACCCAGGAAG-3′	5′-TGCTGGTAGGTAAGTAAGGAG-3′
Reverse primer	5′-GTGGATACATACAAGAGAGACTTTGTCCC-3′	5′-CAAGAGAGACTTTGTCCC-3′	5′-CAAGAGAGACTTTGTCCC-3′
Wild-type probe	/5HEX/CA + C + T + C + T + T + GC/3IABkFQ/	5′-HEX-TC + GC + A + A + GA + GT + GC-IABkFQ-3′	n/a (used only primers for pre-amplification)
Mutant probea	/56-FAM/CA + CT + C + A + T + GCG/3IABkFQ/	5′-6-FAM-TC + GC + A + T + GA + GTGC-IABkFQ-3′
ddPCR Amplicon	173 bp	130 bp	300 bp

HEX hexachlorofluorescein, 6-FAM 6-carboxyfluorescein, IABkFQ Iowa Black FQ quencher.

^aMutant base is bold, “ + ” denotes LNA bases.