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. 2021 Mar 3;11:5057. doi: 10.1038/s41598-021-84600-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation of oligodendrocyte precursors is not changed by RT + aPD-1. (A) Sham operated and tumor implanted mice received daily i.p. injections of BrdU starting at 60 dpi until 67 dpi. Brain coronal sections were collected at 90 dpi and immunostained with specific antibodies for BrdU (red) and the oligodendrocyte marker Olig2 (green). Representative micrographs of the external capsule of the corpus callosum (EC) for the indicated experimental groups are shown. Images were

taken from the contralateral hemisphere (CTL). Arrows indicate double positive cells, scale bar = 50 µm. (B) The number of newly generated oligodendrocytes assessed by Olig2 and BrdU double immunoreactivity was quantified in the EC. n = 3 per group.