Figure 1.

Effects of the culture media on light absorbance and SARS-CoV-2 infectivity. (a) Absorbance spectra of EMEM, FBS, PBS, and their mixed media. The absorbance by the various media indicated was monitored using a UV–visible spectrometer. FBS concentrations in the culture media are shown in parentheses. EMEM (2% FBS)/PBS (2% FBS), red line; EMEM containing 2% FBS diluted with PBS containing 2% FBS by tenfold. (b) Effects of the culture media on viral infectivity. Virus samples were diluted with EMEM containing 2% FBS (EMEM) or PBS containing 2% FBS (PBS). The samples appropriately diluted for plaque assays were inoculated into Vero E6 cells. On day 3 post-infection, cells were fixed and stained to visualize plaques. Representative data from six independent assays are shown.