Fig. 6. Alternative splicing of AR isoforms is present across metastases, as determined by DSP.

a Heatmap of 141 individual tumor ROIs grouped by 26 patients comparing bulk RNAseq AR-V7 expression to DSP. Results are expressed as gene signature Z-scores and log₂ negative-normalized (NN) gene expression and presented according to color scales. b DSP–RNA probe design of AR full length and AR-V7 variant isoform. c Fluorescent labeling and ROI selection of three cores from two sites of metastasis from patients 14-043 that demonstrated divergent AR-V7 expression determined by RNAseq and DSP. N = 1 TMA section for fluorescent labeling. d Heatmap demonstrating discordance in AR-V7 expression DSP AR-V7 and AR-V7 measured by bulk RNAseq and AR-V7 immunohistochemistry (IHC) in six tumor ROIs from two sites (CC3 and H3) within patient 14-043. Results are presented according to color scales in a.