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. 2021 Feb 18;8:618574. doi: 10.3389/fcell.2020.618574

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Copyright © 2021 Cheng, Wang, Wu, Cui, Wei, Liu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The depletion of circPostn attenuates cardiac remodeling in MI mice (Rzem et al., 2007). The left ventricular chamber of 3-day post-MI mice was injected with the lentiviral vector of control shRNA or circPostn shRNA. (A) The mRNA expression of collagen 1α1 and collagen 3α1 was measured by qPCR in mice. (B) The protein expression of collagen and α-SMA was tested by western blot analysis in mice. The results of western blot analysis were quantified by ImageJ software. (C) The protein expression of ANP and BNP was assessed by western blot analysis in mice. The results of western blot analysis were quantified by ImageJ software. (D) The protein expression of Bcl-2, Bax, cleaved caspase-3, and β-actin was assessed by western blot analysis in mice. The results of western blot analysis were quantified by ImageJ software. Data are presented as mean ± SD. Statistically significant differences were indicated: *p < 0.05, **p < 0.01, ^##p < 0.01.