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. 2021 Feb 18;8:618574. doi: 10.3389/fcell.2020.618574

FIGURE 4.

FIGURE 4

circPostn serves as a miR-96-5p sponge in human cardiomyocytes. (A) The potential interaction between circPostn and miR-96-5p was identified in a bioinformatic analysis by using ENCORI (see text footnote 1). (B) The expression levels of miR-96-5p were assessed by qPCR in the H/R-treated AC16 cells. (C) The expression levels of miR-96-5p were measured by qPCR in the AC16 cells treated with control mimic or miR-96-5p mimic. (D) The luciferase activities of wild-type circPostn (circPostn WT) and circPostn with the miR-96-5p-binding site mutant (circPostn MUT) were determined by luciferase reporter gene assays in the AC16 cells treated with control mimic or miR-96-5p mimic. (E,F) The AC16 cells treated with control shRNA or circPostn shRNA. (E) The expression of circPostn was measured by qPCR in mice. (F) The expression of miR-96-5p was measured by qPCR in the AC16 cells. (G–J) The left ventricular chamber of 3-day post-MI mice was injected with the control mimic or miR-96-5p mimic. (G) The mRNA expression of collagen 1α1 and collagen 3α1 was measured by qPCR in mice. (H) The protein expression of collagen and α-SMA was tested by western blot analysis in mice. The results of western blot analysis were quantified by ImageJ software. (I) The protein expression of ANP and BNP was assessed by western blot analysis in mice. The results of western blot analysis were quantified by ImageJ software. (J) The protein expression of Bcl-2, Bax, cleaved caspase-3, and β-actin was assessed by western blot analysis in mice. The results of western blot analysis were quantified by ImageJ software. Data are presented as mean ± SD. Statistically significant differences were indicated: *p < 0.05, **p < 0.01.