FIGURE 2.

Deletion of Mettl3 in K14⁺ cells displays reduced tumorigenicity, cell proliferation, and survival. (A) Representative images of bladder cancer (BCa) lesions from K14^CreER, Mettl3^wt/wt or K14^CreER, Mettl3^flox/flox mice. (B) The bladder weight was calculated in wild-type and Mettl3^flox/flox mice, respectively. (C) Representative H&E staining images of BCa section in wild-type and Mettl3^flox/flox mice show the histological atypia. Scale bars, 50 μm. Scale bar, 100 μm. (D) Assessment of malignancy in wild-type and Mettl3^flox/flox mice by tumor grading. (E) Immunofluorescent staining was used to evaluate the expression of K14, Mettl3 (left), Ki67 (middle), and Caspase-3 (right). 4′,6-Diamidino-2-phenylindole (DAPI) staining was used to mark nucleus. Scale bar, 100 μm. (F) Calculation of Mettl3 (left), Ki67 (middle), and Caspase-3 (right) positive cells in wild-type and K14^CreER, Mettl3^flox/flox mice. (G) Immunostaining and IHC score of AKT1 and BCL9L in K14CreER wild type and K14CreER KO mice respectively.