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. 2021 Feb 18;9:639851. doi: 10.3389/fcell.2021.639851

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Copyright © 2021 Zhao, Gao, Liang, Chen, Wang, Wang, Xiao, Zhao, Wan, Jiang, Luo, Wang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of proteins involved in oxidative stress. (A–F) The expression of proteins involved in oxidative stress detected by Western blot (n = 3). (G) The content of Fe²⁺ in the RPE-choroidal complex after CNV (n = 6). (H) Cell death (white arrow) after laser by PI staining. (I) The level of lipid peroxidation (white arrow) after laser with C11 BODIPY 581/591. (J) The expression of Nrf2 mRNA after laser by RT-PCR (n = 6). Bar graphs show mean ± SD. P-values: t-test and one-way ANOVA analysis (***p < 0.001, **p < 0.01, *p < 0.05). Scale bar = 50 μm.