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. 2021 Feb 18;9:639851. doi: 10.3389/fcell.2021.639851

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Copyright © 2021 Zhao, Gao, Liang, Chen, Wang, Wang, Xiao, Zhao, Wan, Jiang, Luo, Wang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The cell viability of SLC7A11^OE ARPE19. (A–C) SLC7A11 and NRF2 protein in SLC7A11^OE ARPE19 were evaluated by Western blot (n = 3). (D–F) SLC7A11, NRF2, and VEGFA mRNA in SLC7A11^OE ARPE19 were evaluated by RT-PCR (n = 6). (G,H) The cell viability of SLC7A11^OE ARPE19 with the stimulation of erastin (200 μM) or RSL3 (20 μM) (n = 5). (I) The cell viability of anti-VEGF treatment and SLC7A11^OE ARPE19 with the stimulation of erastin (200 μM) or RSL3 (20 μM) (n = 5). Bar graphs show mean ± SD. P-values: one-way ANOVA analysis and t-test (***p < 0.001, **p < 0.01, *p < 0.05).