FIGURE 5.

A. fumigatus-induced EETs release involves Akt and p38-MAPK signaling pathways. Human eosinophils were pretreated with (A,C) an Akt inhibitor (iAkt VIII – 2.6 μM) and (B,C) a p38-MAPK inhibitor (SB202190 – 10 μM) or their vehicles (DMSO) for 30 min and were subsequently stimulated with A. fumigatus conidia (cell: fungus ratio of 1:10) for 6 h. (A,B) EETs were quantified in the samples using a fluorescence microplate reader after Sytox Green addition (5 μM). The graphs represent the mean ± SEM of 3 (A) and 4 (B) independent experiments from different donors. *P < 0.05 compared to the non-stimulated sample (medium); # P < 0.05 compared to AF + vehicle; one-way ANOVA followed by the Newman-Keuls test. Samples were analyzed by (C) confocal fluorescence microscopy after staining for DNA (Sytox Green, green). Representative images of 3 (iAkt) and 4 (SB202190) independent experiments (n = 3 and n = 4). AF = Aspergillus fumigatus.