FIGURE 6.

A. fumigatus-induced release of EETs depends on calcium. Human eosinophils were pretreated with the calcium chelator BAPTA-AM (10 μM) or its vehicle (DMSO) for 30 min and were subsequently stimulated with A. fumigatus conidia (cell: fungus ratio of 1:10) for 6 h. (A) EETs were quantified in the samples using a fluorescence microplate reader after Sytox Green addition (5 μM). The graph represents the mean ± SEM of 3 independent experiments from different donors. *P < 0.05 compared to the non-stimulated sample (medium); #P < 0.05 compared to AF + vehicle; one-way ANOVA followed by the Newman-Keuls test. (B) Confocal fluorescence microscopy of human eosinophils pretreated with BAPTA-AM (10 μM) or its vehicle (DMSO) for 30 min and subsequently stimulated with A. fumigatus conidia (cell: fungus ratio of 1:10) for 6 h after staining for DNA (Sytox Green, green). Representative images of 4 independent experiments (n = 4). AF = Aspergillus fumigatus.