Skip to main content
. 2021 Feb 18;9:624312. doi: 10.3389/fcell.2021.624312

FIGURE 2.

FIGURE 2

Schematics for FRET biosensors. (A) Spectra of YPET and ECFP. The spectra of YPET and ECFP, a popular FP pair for FRET biosensor, is shown here. A large overlap between the emission spectrum of the donor and the excitation spectrum of the acceptor is crucial for the success of a FRET biosensor. (B) Distance and orientation of FRET pair. A short distance and correct orientation (not orthogonal) are necessary for the efficiency of the energy transfer. (C) A schematic for an intermolecular FRET biosensor that detects binding of two domains. (D) A schematic for an intramolecular FRET biosensor that detects change of conformation of one domain. (E) A schematic for an intermolecular FRET biosensor.