FIGURE 2.

CD39 and CD73 ectonucleotidases are upregulated during an in vivo immune response. Naive OT-I cells (10⁶ cells) were injected into CD45.1+ recipient mice. 24 h later, the recipient mice were immunized i.p with OVA protein (500 μg) + LPS (25 μg). Transferred cells were analyzed in blood samples on days 4, 7, 12, 21, and 28 after the immunization. (A–D) CD39 and CD73 expression was analyzed in the effector memory precursors (CD45.2+/CD8+/CD44+/CD62L–) or central memory precursors (CD45.2+/CD8+/CD44+/CD62L+) (E–H) at different time points. n = 4, Kruskal–Wallis Test, *p < 0.05; **p < 0.01.