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Optimization and validation of Plectin-1 in healthy controls. To validate the specificity of Plectin-1 antigen in detecting circulating tumor cells (CTCs), the number of CTCs was counted in peripheral blood samples of eighteen healthy volunteers after immunostaining with anti-Plectin-1 and anti-CD45 antibody. Plectin-1+CD45-DAPI+ CTC counts per 1 ml blood were calculated individually.
