Figure 5.

Iron content in db/m, db/db, and db/db+Fer1 groups. (A, B). Representative photographs and quantification of iron content (blue) in kidneys by Lillie staining (six sections per mouse were analyzed). (C). Representative photographs and quantification of ferritin heavy chain in mouse kidneys measured by western blot. (D–F). Quantification of serum iron ion (D), ferritin (E), and transferrin (F) measured by ELISA. Male mice, n = 6–9/group. *P < 0.05, vs db/m group; ^# P < 0.05, vs db/db group. Db/m, db/m mice; db/db, db/db mice without Ferrostatin-1 treatment; db/db+Fer1, db/db mice with Ferrostatin-1 treatment. Data are means ± S.E.M.