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. 2021 Feb 18;9:634056. doi: 10.3389/fcell.2021.634056

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Copyright © 2021 Luo, Dong, Yu, Xia, Berry, Rao, Xu, Xue, Chen, Lin, Yu, Huang, Li, Zhou and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ZNF124 is specifically required for the growth of the aggressive G3MB cell. (A) Survival (5 years) analysis of the driver gene based on the ZNF124 expression using the publicly available datasets (GEO: gse85217). (B) RT-PCR experiment to detect ZNF124 mRNA (upper) and Western blotting to exam ZNF124 protein (lower) expression in human MB cell lines. Data represent means ± SD, n = 3 independent experiments. (C) Fold change in proliferation (as measured by WST-1) of control and shZNF124-expressing D458 cells. Values are means ± SD of five independent measurements across three replicate experiments. **p < 0.01, two-tailed unpaired t-test. (D) BrdU staining images for the control and shZNF124 D425 cells (left) and the quantification (right). Data represent means ± SD, n = 3 independent experiments. *p < 0.05; **p < 0.01, two-tailed unpaired t-test.