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. 2021 Feb 18;9:634056. doi: 10.3389/fcell.2021.634056

FIGURE 6.

FIGURE 6

Knockdown ZNF124 promotes apoptosis of G3-MB cells. (A) RT-PCR data showed the efficiency of shZNF124 in MYC amplificated G3-MB D458 cells and the cell cycle inhibitor gene CDKN1A/P21, apoptosis genes BAX and PUMA and tumor stemness gene MYC and SOX2 mRNA expression. Data represent means ± SD, n = 3 independent experiments. **p < 0.01, two-tailed unpaired t-test. (B) Western blotting analysis of MYC and ZNF124 protein expression in human MB D458 cell after ZNF124 knockdown with Lenti-shRNA for 72 h. Ratio of MYC expression over GAPDH control was shown. (C,D) Dying cells were detected by flow cytometry in G3-D458 and SHH-DAOY cells after knockdown ZNF124 (C) and the comparison of the dead cells in control and shZNF124 treatment cells (D). Data represent means ± SD, n = 3 independent experiments. **p < 0.01, two-tailed unpaired t-test.