Figure 3. Knockdown of LRG1 prevented EMT of ARPE-19 cells.

A: By reverse transcription quantitative PCR, the expressions of fibronectin and α-SMA were reduced, meanwhile the mRNA expression level of ZO-1 was increased when ARPE-19 cells were treated with siRNA of LRG1. B: By Western blot analysis, when using siRNA of LRG1 in ARPE-19 cells, the protein expressions of fibronectin and α-SMA were down-regulated, and the ZO-1 expression was up-regulated. ^bP<0.01; ^cP<0.001.