Figure 3.

In vitro kinase assay with purified Fu and detection with pIMAGO

In vitro kinase assay using purified CC-Fu^EE or CC-Fu^GV as source of kinase and GST-fusion proteins containing either CiN (A) or CiC (B) fragment with the wild-type sequence or the indicated amino acid substitutions. Phosphorylation was detected by the pIMAGO kit. GST-fusion proteins were visualized by Coomassie blue gel staining (CB). The Fu phosphorylation sites are colored in red and the critical acid residues at +6 position are colored in blue. Figure reprinted with permission from Han et al. (2019).