| Antibodies |
|
| Anti-Ci pS218/S220 |
Abmart |
N/A |
| Anti-Ci pS1230/S1233 |
Abmart |
N/A |
| IRDye 680LT donkey anti-rabbit secondary antibody |
LI-COR |
Cat#926-68023 |
|
| Bacterial and virus strains |
|
| BL21 chemically competent E. coli
|
Thermo Fisher Scientific |
Cat#EC0114 |
| MAX efficiency DH10Bac chemically competent cells |
Thermo Fisher Scientific |
Cat#10361012 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Gentamycin |
Millipore Sigma |
Cat#G1397 |
| Ampicillin |
Millipore Sigma |
Cat#A5354 |
| Kanamycin |
Millipore Sigma |
Cat#60615 |
| Tetracycline |
Millipore Sigma |
Cat#T7660 |
| IPTG |
Millipore Sigma |
Cat#I6758 |
| Bluo-Gal |
Thermo Fisher Scientific |
Cat#15519028 |
| L-Glutathione reduced |
Millipore Sigma |
Cat#G4251 |
| Sf-900 III SFM |
Thermo Fisher Scientific |
Cat#12658027 |
| 3X Flag peptide |
Millipore Sigma |
Cat#F4799 |
| Fetal bovine serum |
Millipore Sigma |
Cat#F4135 |
| Complete EDTA-free protease inhibitor cocktail |
Roche |
Cat#04 693 159 001 |
| PhosSTOP phosphatase inhibitor cocktail |
Roche |
Cat#04 906 837 001 |
| Casein kinase I |
New ENGLAND BIOLABS |
Cat#P6030 |
|
| Critical commercial assays |
|
| pIMAGO-biotin Phosphoprotein Detection Kit |
Millipore Sigma |
Cat#67221 |
| Anti-Flag M2 affinity gel |
Millipore Sigma |
Cat#A2220 |
| Glutathione Sepharose 4B |
GE Healthcare |
Cat#17075601 |
| SimplyBlue SafeStain |
Thermo Fisher Scientific |
Cat#LC6060 |
| Cellfectin II Reagent |
Thermo Fisher Scientific |
Cat#10362100 |
| Amicon Ultra-0.5 mL centrifugal filters |
Millipore Sigma |
Cat#UFC501024 |
|
| Experimental models: cell lines |
|
| Insect: Sf9 |
Thermo Fisher Scientific |
Cat#11496015 |
|
| Recombinant DNA |
|
| pGEX 4T-1 |
GE Healthcare |
Cat#28954549 |
| pFastBac1 |
Thermo Fisher Scientific |
Cat#10359016 |