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. 2021 Mar 1;2(1):100376. doi: 10.1016/j.xpro.2021.100376
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-Ci pS218/S220 Abmart N/A
Anti-Ci pS1230/S1233 Abmart N/A
IRDye 680LT donkey anti-rabbit secondary antibody LI-COR Cat#926-68023

Bacterial and virus strains

BL21 chemically competent E. coli Thermo Fisher Scientific Cat#EC0114
MAX efficiency DH10Bac chemically competent cells Thermo Fisher Scientific Cat#10361012

Chemicals, peptides, and recombinant proteins

Gentamycin Millipore Sigma Cat#G1397
Ampicillin Millipore Sigma Cat#A5354
Kanamycin Millipore Sigma Cat#60615
Tetracycline Millipore Sigma Cat#T7660
IPTG Millipore Sigma Cat#I6758
Bluo-Gal Thermo Fisher Scientific Cat#15519028
L-Glutathione reduced Millipore Sigma Cat#G4251
Sf-900 III SFM Thermo Fisher Scientific Cat#12658027
3X Flag peptide Millipore Sigma Cat#F4799
Fetal bovine serum Millipore Sigma Cat#F4135
Complete EDTA-free protease inhibitor cocktail Roche Cat#04 693 159 001
PhosSTOP phosphatase inhibitor cocktail Roche Cat#04 906 837 001
Casein kinase I New ENGLAND BIOLABS Cat#P6030

Critical commercial assays

pIMAGO-biotin Phosphoprotein Detection Kit Millipore Sigma Cat#67221
Anti-Flag M2 affinity gel Millipore Sigma Cat#A2220
Glutathione Sepharose 4B GE Healthcare Cat#17075601
SimplyBlue SafeStain Thermo Fisher Scientific Cat#LC6060
Cellfectin II Reagent Thermo Fisher Scientific Cat#10362100
Amicon Ultra-0.5 mL centrifugal filters Millipore Sigma Cat#UFC501024

Experimental models: cell lines

Insect: Sf9 Thermo Fisher Scientific Cat#11496015

Recombinant DNA

pGEX 4T-1 GE Healthcare Cat#28954549
pFastBac1 Thermo Fisher Scientific Cat#10359016