FIGURE 5.

Genetic ablation of 5-LO ameliorated liver fibrosis after CCl₄ injection. WT and 5-LO ^−/− mice were treated with olive oil or CCl₄ for 8 weeks. Fibrosis stage was assessed by picrosirius red staining (A,B) for collagen according to the Ishak criteria (C). (D) Detection of hepatic hydroxyproline level. (E,F) qRT-PCR analysis of mRNA levels of fibrosis genes in livers of 4 groups. (G) Western blot analysis of the protein levels of α-SMA and Col1a1. Data are mean ± SEM, n = 7, *p < 0.05, **p < 0.01.