FIGURE 7.
Targeted delivery of zileuton to HSC is efficient against CCl4-induced liver fibrosis. WT mice were treated with olive oil or CCl4 for 8 weeks. During the last 4 weeks, mice given RGD-Lip or RGD-Lip/zileuton (10 mg/kg) every 3 days by tail vein injection. (A,B) Liver sections were collected for picrosirius red staining. (C) Fibrosis score was assessed for collagen according to the Ishak criteria. (D) Detection of hepatic hydroxyproline level. (E,F) qRT-PCR analysis of mRNA levels of fibrotic genes in livers of 4 groups. (G) Western blot analysis of the protein levels of α-SMA and Col1a1. Data are mean ± SEM, n = 7, *p < 0.05, **p < 0.01.