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. 2021 Feb 18;12:619950. doi: 10.3389/fgene.2021.619950

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Copyright © 2021 Diroma, Modi, Lari, Sineo, Caramelli and Vai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mitochondrial phylogenetic trees from Polizzello sequences. Phylogenetic trees of mitochondrial DNA variation in Polizzello samples were obtained by Haplogrep2 from consensus sequences generated by schmutzi (A) and those obtained from variants called by Mutect2 and retained after filtering based on allele depth (≥10) and allele fraction (≥50%) and considering the highest contamination rate (2%) (B). Slightly different haplogroups were predicted for three samples (Pol-3, Pol-10, Pol-29). Amino acid changes are shown for non-synonymous variants.