TABLE 5.

Physicochemical properties and drug loading of andrographolide nanoformulation with different drug-polymer ratio.

Formulation code	Particle size (nm)	Polydispersity index–PI	Zeta potential (mV)	Drug loading (%)	Encapsulation efficiency (%)
D1	1444***	0.180.03	−11.31.2	Unquantifiable	Unquantifiable
D2	1485***	0.200.02	−8.21.2	1.00.0***	13.90.4
D3	1332**	0.200.03	−10.12.0	2.20.3	23.23.7
D4	1126	0.200.02	−11.410	2.30.3	18.22.1
D5	1309*	0.210.04	−8.12.3	1.80.3	9.81.8**

Results are expressed as mean ± SD (n = 3). *signifies p < 0.05, **signifies p < 0.01, ***signifies p < 0.001 significant differences with respect to D4 formulation. D1, 1:20; D2, 1:12; D3, 1:8.5; D4, 1:6; D5, 1:4.