Table 3.
Intraday and interday accuracy and precision of the 18 anthelmintics in chicken, pork, beef, milk, and egg.
| Compound | Spiking level | Intraday accuracy (%) (%RSD) (n = 3) |
Interday accuracy (%) (%RSD) (n = 9) |
R2 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chicken | Pork | Beef | Milk | Egg | Chicken | Pork | Beef | Milk | Egg | |||
| Closantel | A | 112.6 (12.7) | 92.6 (9.1) | 67.6 (7.5) | 112.6 (11.3) | 85.3 (4.7) | 105.0 (16.0) | 100.7 (14.5) | 64.9 (3.7) | 70.7 (8.6) | 77.0 (15.1) | 0.9958 |
| B | 111.2 (1.8) | 103.7 (7.7) | 92.3 (11.0) | 111.2 (1.6) | 93.4 (1.5) | 103.5 (9.3) | 99.1 (7.1) | 85.5 (9.5) | 91.1 (15.7) | 95.3 (7.3) | ||
| C | 108.8 (6.3) | 107.9 (9.2) | 103.3 (12.1) | 108.8 (5.8) | 109.3 (7.3) | 107.1 (1.3) | 103.5 (9.3) | 94.6 (16.5) | 91.8 (17.5) | 98.3 (12.7) | ||
| Nitroxynil | A | 73.8 (2.3) | 108.3 (2.0) | 81.2 (12.5) | 73.8 (3.1) | 87.0 (7.0) | 68.8 (6.8) | 88.9 (19.9) | 71.8 (15.9) | 70.8 (11.6) | 79.0 (8.8) | 0.9914 |
| B | 91.6 (2.0) | 108.1 (8.0) | 109.5 (11.8) | 91.6 (2.2) | 93.5 (9.1) | 87.1 (7.1) | 99.5 (12.8) | 96.7 (11.6) | 82.6 (14.7) | 90.5 (4.5) | ||
| C | 95.8 (1.2) | 118.4 (5.5) | 105.5 (1.6) | 95.8 (1.3) | 98.7 (8.5) | 87.1 (7.1) | 110.6 (14.8) | 105.7 (1.3) | 96.6 (4.1) | 91.8 (6.6) | ||
| Niclosamide | A | 64.6 (2.1) | 91.5 (4.9) | 81.9 (9.8) | 64.6 (3.2) | 91.5 (2.8) | 68.2 (9.1) | 92.5 (15.1) | 74.4 (18.0) | 95.4 (7.3) | 87.5 (5.9) | 0.9974 |
| B | 78.3 (7.7) | 109.1 (15.3) | 70.9 (2.8) | 78.3 (9.8) | 112.6 (1.0) | 72.2 (5.4) | 109.8 (6.1) | 70.8 (4.2) | 93.4 (10.8) | 114.0 (1.8) | ||
| C | 83.3 (0.7) | 111.8 (5.0) | 78.6 (7.1) | 83.3 (0.9) | 115.0 (2.9) | 80.4 (2.3) | 117.9 (8.3) | 81.9 (12.2) | 95.4 (6.9) | 113.3 (2.4) | ||
| Rafoxanide | A | 83.8 (1.7) | 72.0 (16.1) | 64.0 (2.8) | 83.8 (2.1) | 73.5 (5.1) | 87.7 (15.7) | 75.4 (14.9) | 65.4 (3.4) | 61.2 (5.0) | 71.8 (4.5) | 0.9955 |
| B | 92.9 (2.0) | 93.4 (9.0) | 71.5 (3.6) | 92.9 (2.2) | 80.6 (5.1) | 95.6 (3.2) | 86.5 (12.9) | 72.2 (12.0) | 72.0 (3.3) | 78.5 (2.4) | ||
| C | 96.7 (6.1) | 112.6 (6.2) | 73.8 (8.9) | 96.7 (6.3) | 72.4 (1.8) | 100.7 (6.9) | 110.9 (2.3) | 70.5 (5.1) | 78.3 (3.3) | 71.2 (3.1) | ||
| Eprinomectin | A | 104.0 (2.8) | 110.3 (5.9) | 68.0 (10.4) | 104.0 (2.7) | 73.9 (7.3) | 103.2 (3.6) | 90.0 (19.9) | 67.8 (8.8) | 83.3 (8.7) | 72.8 (15.3) | 0.9816 |
| B | 79.0 (6.5) | 103.1 (1.4) | 90.8 (11.9) | 79.0 (8.3) | 84.4 (2.7) | 91.2 (11.6) | 86.2 (17.5) | 80.9 (13.6) | 87.1 (8.0) | 83.9 (6.6) | ||
| C | 70.0 (2.0) | 84.2 (6.6) | 91.9 (4.0) | 70.0 (2.9) | 73.8 (1.8) | 73.5 (4.8) | 75.9 (9.6) | 84.6 (9.3) | 84.1 (7.1) | 73.6 (3.8) | ||
| Emamectin | A | 85.4 (5.1) | 77.0 (8.1) | 66.1 (3.2) | 85.4 (6.0) | 80.0 (10.5) | 81.9 (12.9) | 86.8 (6.6) | 64.04 (3.9) | 77.4 (10.9) | 74.4 (11.0) | 0.9978 |
| B | 81.8 (4.3) | 91.4 (10.8) | 73.4 (11.3) | 81.8 (5.3) | 86.1 (7.9) | 76.3 (6.3) | 86.8 (13.4) | 70.1 (9.8) | 79.7 (8.8) | 97.2 (10.3) | ||
| C | 78.3 (3.2) | 104.1 (11.4) | 80.6 (8.5) | 78.3 (4.1) | 106.4 (5.9) | 83.3 (8.0) | 103.4 (4.1) | 77.0 (4.8) | 77.2 (4.8) | 115.9 (7.5) | ||
| Levamisole | A | 70.2 (7.8) | 80.6 (15.0) | 80.6 (6.9) | 70.2 (1.0) | 65.0 (7.4) | 64.3 (8.0) | 81.8 (1.9) | 70.0 (8.9) | 84.9 (19.8) | 71.1 (9.5) | 0.9964 |
| B | 74.6 (7.5) | 88.6 (12.2) | 86.4 (7.2) | 74.6 (9.9) | 74.0 (12.4) | 73.8 (1.7) | 84.2 (4.5) | 80.6 (6.2) | 78.2 (13.6) | 73.8 (6.0) | ||
| C | 81.5 (0.6) | 86.7 (8.9) | 78.5 (7.7) | 81.5 (0.6) | 76.5 (6.0) | 86.7 (5.6) | 83.0 (4.3) | 75.1 (4.0) | 78.2 (8.9) | 80.6 (4.8) | ||
| Cymiazole | A | 78.5 (2.9) | 72.4 (7.2) | 67.6 (10.1) | 78.5 (2.9) | 78.0 (17.3) | 74.8 (4.9) | 65.4 (9.7) | 70.2 (4.2) | 105.5 (19.1) | 81.6 (15.0) | 0.9973 |
| B | 79.4 (6.7) | 79.3 (6.8) | 74.9 (9.4) | 79.4 (6.7) | 91.2 (7.1) | 74.6 (6.2) | 72.7 (10.3) | 68.5 (8.1) | 95.0 (18.3) | 85.0 (11.4) | ||
| C | 75.2 (5.1) | 76.1 (2.8) | 76.2 (6.5) | 75.2 (5.1) | 96.1 (4.2) | 72.6 (4.1) | 72.0 (7.3) | 75.6 (8.1) | 87.7 (11.7) | 87.0 (10.8) | ||
| Praziquantel | A | 79.8 (4.0) | 88.6 (9.5) | 72.5 (4.9) | 79.7 (9.9) | 88.3 (2.7) | 77.0 (6.7) | 96.1 (17.1) | 70.3 (4.4) | 88.8 (8.2) | 87.7 (1.9) | 0.9985 |
| B | 85.2 (3.4) | 89.0 (6.1) | 79.4 (6.3) | 85.5 (3.0) | 107.6 (8.6) | 85.1 (2.3) | 93.2 (11.1) | 75.9 (6.1) | 89.8 (9.1) | 101.8 (13.3) | ||
| C | 85.4 (3.4) | 106.3 (12.0) | 78.2 (3.9) | 85.4 (4.0) | 104.1 (3.4) | 82.0 (3.8) | 95.3 (12.0) | 77.2 (8.4) | 87.2 (7.5) | 101.8 (9.0) | ||
| Tetramisole | A | 101.0 (10.8) | 75.9 (14.3) | 103.9 (9.8) | 101.0 (10.7) | 88.4 (10.9) | 100.7 (7.4) | 92.7 (17.3) | 96.1 (7.2) | 68.5 (12.9) | 88.4 (7.0) | 0.9931 |
| B | 86.5 (11.6) | 74.9 (6.9) | 92.0 (12.5) | 86.5 (13.4) | 83.9 (6.5) | 86.5 (15.0) | 85.6 (13.3) | 89.7 (3.2) | 70.7 (10.5) | 81.8 (9.1) | ||
| C | 89.9 (9.8) | 86.4 (5.7) | 84.1 (6.4) | 89.85 (10.9) | 83.4 (8.5) | 80.9 (9.6) | 82.0 (11.9) | 84.1 (6.6) | 97.8 (18.3) | 81.0 (3.7) | ||
| Thiophanate | A | 89.3 (4.7) | 89.6 (3.9) | 88.0 (4.0) | 89.3 (5.2) | 110.3 (0.9) | 85.5 (14.6) | 73.3 (19.2) | 81.9 (9.2) | 94.3 (3.5) | 113.5 (2.4) | 0.9999 |
| B | 91.0 (3.5) | 90.7 (9.3) | 92.0 (12.5) | 91.0 (3.9) | 104.2 (0.9) | 90.2 (5.0) | 87.8 (7.9) | 87.8 (9.4) | 70.8 (11.3) | 104.3 (7.8) | ||
| C | 93.8 (1.7) | 99.2 (10.6) | 84.1 (6.4) | 93.8 (1.8) | 98.2 (5.2) | 90.9 (4.2) | 88.2 (14.2) | 92.8 (5.2) | 72.5 (4.1) | 96.0 (4.3) | ||
| Morantel | A | 73.8 (6.4) | 80.6 (2.6) | 93.2 (10.2) | 73.8 (8.7) | 101.9 (3.1) | 75.5 (16.3) | 83.0 (10.7) | 81.5 (12.5) | 100.8 (1.3) | 101.2 (1.9) | 0.9979 |
| B | 85.5 (2.6) | 89.4 (13.3) | 80.6 (5.5) | 85.5 (3.0) | 94.8 (6.2) | 89.86 (18.9) | 89.8 (7.1) | 80.5 (1.8) | 99.7 (2.6) | 97.1 (6.1) | ||
| C | 94.8 (7.4) | 97.6 (6.9) | 93.4 (8.7) | 94.8 (7.8) | 99.6 (3.8) | 94.0 (12.1) | 84.5 (14.6) | 90.5 (4.2) | 103.8 (10.2) | 102.7 (4.6) | ||
| Pyrantel | A | 86.9 (6.3) | 78.1 (8.3) | 83.2 (16.0) | 86.9 (7.3) | 90.3 (3.4) | 76.1 (13.6) | 79.0 (13.5) | 86.4 (12.9) | 83.8 (10.4) | 100.2 (8.9) | 0.9981 |
| B | 111.0 (7.5) | 87.6 (10.8) | 92.4 (13.1) | 111.0 (6.8) | 96.9 (4.1) | 104.6 (16.1) | 81.6 (8.4) | 88.2 (18.1) | 77.6 (4.0) | 98.8 (3.1) | ||
| C | 88.8 (11.6) | 95.2 (8.7) | 81.2 (6.0) | 88.8 (13.0) | 98.1 (5.8) | 93.0 (8.0) | 83.4 (11.5) | 80.3 (5.6) | 86.5 (9.8) | 98.4 (6.0) | ||
| Fluazuron | A | 99.8 (9.1) | 74.3 (15.5) | 74.7 (8.5) | 99.8 (9.1) | 112.1 (6.9) | 97.2 (9.1) | 75.4 (17.7) | 80.0 (11.0) | 106.1 (8.3) | 110.6 (6.1) | 0.9950 |
| B | 73.2 (4.6) | 108.7 (4.6) | 70.5 (0.5) | 73.2 (6.2) | 116.1 (5.1) | 74.2 (2.3) | 103.1 (7.9) | 85.9 (16.5) | 106.5 (9.4) | 110.1 (6.2) | ||
| C | 72.2 (6,2) | 110.9 (11.5) | 91.5 (4.8) | 72.2 (8.6) | 104.7 (1.8) | 71.7 (2.5) | 106.7 (8.1) | 91.9 (5.4) | 104.6 (8.0) | 108.8 (5.6) | ||
| Guaifenesin | A | 84.2 (9.9) | 100.1 (10.0) | 108.3 (4.7) | 84.2 (11.7) | 85.9 (9.5) | 76.0 (11.4) | 92.9 (16.0) | 105.4 (15.4) | 82.9 (12.5) | 79.7 (14.9) | 0.9799 |
| B | 82.6 (1.9) | 106.1 (8.5) | 86.1 (6.9) | 82.6 (10.4) | 73.5 (5.9) | 84.8 (17.7) | 99.2 (6.6) | 77.2 (10.9) | 88.9 (4.4) | 84.6 (13.5) | ||
| C | 93.6 (1.9) | 96.2 (7.0) | 81.8 (4.6) | 93.6 (2.1) | 103.4 (11.7) | 86.8 (11.8) | 88.2 (8.0) | 82.2 (6.2) | 84.2 (1.7) | 101.5 (12.6) | ||
| Carbendazim | A | 88.9 (8.6) | 95.1 (8.4) | 90.0 (3.6) | 88.9 (9.6) | 106.5 (6.9) | 81.3 (8.1) | 87.3 (8.9) | 90.7 (6.4) | 95.6 (10.2) | 113.9 (6.4) | 0.9946 |
| B | 86.6 (3.5) | 93.4 (9.9) | 97.1 (6.0) | 86.6 (4.1) | 117.9 (11.7) | 86.0 (2.4) | 97.9 (18.8) | 85.2 (12.7) | 95.2 (15.7) | 113.9 (6.0) | ||
| C | 83.9 (3.4) | 106.9 (3.2) | 92.6 (2.1) | 83.9 (4.0) | 110.0 (5.7) | 86.2 (9.9) | 98.0 (18.6) | 92.1 (1.6) | 91.6 (18.2) | 104.4 (8.7) | ||
| Cambendazole | A | 90.8 (5.9) | 77.0 (16.7) | 103.4 (11.9) | 90.8 (6.5) | 88.1 (2.4) | 81.6 (10.0) | 74.3 (13.9) | 99.2 (7.7) | 71.7 (9.1) | 88.1 (0.2) | 0.9996 |
| B | 99.4 (0.6) | 84.5 (4.3) | 110.3 (11.8) | 99.4 (0.6) | 93.1 (2.7) | 93.8 (5.2) | 77.5 (16.8) | 103.6 (6.7) | 76.2 (4.0) | 91.5 (2.3) | ||
| C | 97.0 (1.3) | 96.5 (8.2) | 111.7 (5.0) | 97.0 (1.4) | 91.3 (7.5) | 95.4 (3.0) | 94.8 (8.3) | 108.5 (7.0) | 76.7 (2.9) | 90.9 (5.1) | ||
| Trichlorfon | A | 91.2 (9.7) | 95.9 (12.7) | 91.0 (2.2) | 91.2 (10.6) | 109.3 (2.4) | 87.0 (8.6) | 88.6 (8.3) | 95.9 (6.6) | 95.9 (12.5) | 103.0 (5.6) | 0.9981 |
| B | 101.7 (4.1) | 88.0 (10.6) | 95.4 (8.6) | 101.7 (4.0) | 101.8 (7.9) | 87.9 (16.4) | 90.1 (2.7) | 88.9 (7.2) | 89.6 (11.0 | 98.4 (12.9) | ||
| C | 81.4 (5.4) | 99.5 (3.7) | 91.9 (8.2) | 81.4 (6.6) | 91.2 (12.9) | 84.1 (6.4) | 93.9 (5.5) | 89.0 (4.7) | 87.6 (8.2) | 89.9 (9.3) | ||
A: MRL ×1/2; B: MRL ×1; C: MRL ×2; MRL: maximum residue limit.