FIGURE 1.

Icariin improves the survival rate of gentamicin-treated HEI-OC1 cells. (A) HEI-OC1 cells were treated with increasing concentrations of gentamicin (0, 5, 50, 500, and 1,000 μM) for 12 h. The survival of HEI-OC1 cells significantly decreased following treatment with 500 μM gentamicin. (B) Quantification of cell viability. Scale bar = 100 μM. (C) HEI-OC1 cells were pretreated with increasing concentrations of icariin (0.2, 2, and 20 μM). Cell viability was significantly increased following pretreatment with high concentrations of icariin. (D) Quantification of cell viability. Scale bar = 100 μM. Each experiment was performed three times. Ns, not significant. Scale bar = 100 μM; N = 8 in each group; *p < 0.05; ***p < 0.001; one-way analysis of variance and Tukey’s multiple comparison test.