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. 2021 Feb 18;12:620741. doi: 10.3389/fphar.2021.620741

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Copyright © 2021 Hu and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Icariin inhibits gentamicin-induced apoptosis in HEI-OC1 cells. (A) HEI-OC1 cells were divided into three groups: control, gentamicin, and icariin + gentamicin. The number of TUNEL-positive cells was distinctly lower in the icariin + gentamicin group than in the other groups. Scale bar = 20 μM (B) Measurement of levels of TUNEL-positive cells in (A). (C) Expression changes of the apoptotic markers Bcl-2, Bax, and Caspase-3 were tested with and without icariin pretreatment. β-Tubulin was used as a loading control. (D) Western blot analyses of Bcl-2, Bax, and Caspase-2 proteins with or without icariin pretreatment. Each experiment was performed three times. Ns, not significant; N = 6 in each group; *p < 0.05; ***p < 0.001; one-way analysis of variance and Tukey’s multiple comparison test.