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. 2021 Feb 18;12:620741. doi: 10.3389/fphar.2021.620741

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Copyright © 2021 Hu and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Icariin inhibits production of intracellular reactive oxygen species (ROS). (A) Intracellular ROS production was examined by flow cytometry. Cells were divided into three groups: control, 500 μM gentamicin, and 20 μM icariin + 500 μM gentamicin. Gentamicin-induced elevation of ROS levels was significantly attenuated by icariin pretreatment. (B) Measurement of ROS levels in (A). (C) Intracellular ROS production was observed by immunofluorescence. Cells were pretreated with increasing concentrations of icariin before gentamicin was added. ROS levels significantly decreased with icariin pretreatment. Scale bar = 100 μM. (D) Measurement of ROS levels in (C). Each experiment was performed three times. Ns, not significant; N = 6 in each group; *p < 0.05; ***p < 0.001; one-way analysis of variance and Tukey’s multiple comparison test.