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. 2021 Feb 18;12:620741. doi: 10.3389/fphar.2021.620741

FIGURE 4.

FIGURE 4

Icariin enhanced SIRT3 expression and promoted AMPK phosphorylation activity. (A) We divided cells into five groups: control; 500 μM gentamicin treatment for 12 h; 0.2 μM icariin pretreatment for 24 h then 500 μM gentamicin treatment for 12 h; 2 μM icariin pretreatment for 24 h then 500 μM gentamicin treatment for 12 h; and 20 μM icariin pretreatment for 24 h then 500 μM gentamicin treatment for 12 h. After collecting cells, PGC-1α and SIRT3 expression was measured by western blotting. (B) Quantification of PGC-1α and SIRT3 protein expression. β-Tubulin was used as a loading control. (C) Western blot analyses of p-AMPK and AMPK in HEI-OC1 cells. Experimental groups are divided as previously described. β-Tubulin was used as a loading control. (D) Quantification of AMPK phosphorylation. Each experiment was performed three times. Ns, not significant; N = 6 in each group; *p < 0.05; ***p < 0.001; one-way analysis of variance and Tukey’s multiple comparison test.