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. 2021 Feb 18;12:620741. doi: 10.3389/fphar.2021.620741

FIGURE 6.

FIGURE 6

Inhibition of SIRT3 activity and AMPK signaling pathway attenuated the anti-apoptotic and anti-oxidative stress functions of icariin. (A) We divided HEI-OC1 cells into seven groups: control, 500 μM gentamicin treatment for 12 h; 200 μM icariin pretreatment for 24 h then 500 μM gentamicin treatment for 12 h; 10 μM compound C treatment for 2 h, icariin treatment for 24 h, and then gentamicin treatment for 12 h; 50 μM 3-TYP treatment for 2 h, icariin treatment for 24 h, and then gentamicin treatment for 12 h; 10 μM compound C treatment for 38 h; and 50 μM 3-TYP treatment for 38 h. TUNEL staining was used to determine the apoptosis rate of HEI-OC1 cells. Scale bar = 20 μM. (B) Measurement of the amount of TUNEL-positive cells in (A). (C) Similar experimental groups to those previously described were used. The level of ROS in HEI-OC1 cells was determined by DCFH-DA staining. Scale bar = 100 μM. (D) Measurement of ROS levels in cells in (C). Each experiment was performed three times. Ns, not significant; N = 6 in each group; *p < 0.05; ***p < 0.001; one-way analysis of variance and Tukey’s multiple comparison test.