FIGURE 7.

SIRT3 and AMPK signaling pathway are involved in the protective effect of icariin on gentamicin-damaged cochlear explants. (A) Cochlear explants were dissected from P3 Kunming mice. We divided the cochlear explants into seven groups, control, 500 μM gentamicin treatment for 12 h; 200 μM icariin pretreatment for 24 h then 500 μM gentamicin treatment for 12 h; 10 μM compound C treatment for 2 h, icariin treatment for 24 h, and then gentamicin treatment for 12 h; 50 μM 3-TYP treatment for 2 h, icariin treatment for 24 h, and then gentamicin treatment for 12 h; 10 μM compound C treatment for 38 h; and 50 μM 3-TYP treatment for 38 h. We divided the cochlear tissue cultured in vitro into three parts: base, middle, and apex. Hair cells were labeled with the classic hair cell marker Myosin VIIa (green). Scale bar = 10 μM (B) Quantitative analysis of the number of hair cells in the Organ of Corti. Each experiment was performed three times. Ns, not significant; N = 4 in each group; *p < 0.05; ***p < 0.001; one-way analysis of variance and Tukey’s multiple comparison test.