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. 2021 Feb 18;12:620741. doi: 10.3389/fphar.2021.620741

FIGURE 8.

FIGURE 8

AMPK regulates SIRT3 expression in HEI-OC1 cells. (A) We divided HEI-OC1 cells into four groups: control; 500 μM gentamicin treatment for 12 h; 200 μM icariin treatment for 24 h, then 500 μM gentamicin treatment for 12 h; and 10 μM compound C treatment for 2 h, icariin treatment for 24 h, and then gentamicin treatment for 12 h. Western blotting was used to determine the protein expression of SIRT3 and PGC-1α. β-Tubulin was used as a loading control. (B) Quantitative analysis of SIRT3 protein expression. (C) Quantitative analysis of PGC-1α protein expression. (D) HEI-OC1 cells were divided into four groups: control; 500 μM gentamicin treatment for 12 h; 200 μM icariin treatment for 24 h, then 500 μM gentamicin treatment for 12 h; and 50 μM 3-TYP treatment for 2 h, icariin treatment for 24 h, and then gentamicin treatment for 12 h. Western blotting was used to determine the protein expression of p-AMPK and AMPK. β-Tubulin was used as a loading control. (E) Quantitative analysis of p-AMPK protein expression. Each experiment was performed three times. Ns, not significant; N = 6 in each group; *p < 0.05; ***p < 0.001; one-way analysis of variance and Tukey’s multiple comparison test.