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. 2021 Feb 18;12:635714. doi: 10.3389/fpls.2021.635714

Figure 1.

Figure 1

Plants lacking fully matured complex N-glycans differ in the root hair landscape and are auxin hypersensitive. (A) Primary root length, (B) lateral root density, (C) root hair density, (D) root hair length, and (E) distance between first general and first partially elongated root hair of N-glycosylation mutants and wild-type plants cultivated for 8 days on mock (black boxes), 15 nM BAP (green boxes), or 15 nM NAA (red boxes). Letters indicate significant differences between groups [p ≤ 0.05; (A–C,E) two-way ANOVA with Tukey post hoc test; (D) Kruskal-Wallis test, FDR corrected via Benjamini-Hochberg]. (F) Pictures of wild type (Col-0) and N-glycosylation mutant root tips. Black arrows indicate the first general root hair while white arrows point to the first partially elongated root hair. Scale bars: 500 μm. (G) N-glycan structures of the mutants tested. PR, primary root; LR, lateral root; RH, root hair; Asn, Asparagine; GlcNAc, N-acetylglucosamine.