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mRNA levels of IL-8, IL-6 and VEGF in cell extracts of human macrophages from peripheral blood after anoxia (95% N2, 5%CO2; A–C) and 100 μM CoCl2 (D–F) treatment at different times (hours). Values are expressed as mean ± SD; n = 3, ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; n.s. not significant. Western blotting analysis shows the HIF-1α protein in total extracts after anoxia (G) and CoCl2 (H) treatment of human macrophages at the indicated times.
