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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Cerebellum. 2021 Feb;20(1):41–53. doi: 10.1007/s12311-020-01179-7

Figure 2. Purkinje neuron spike waveform is not prominently affected in 16 week-old SCA3 mice.

Figure 2.

Patch-clamp recordings were performed in vehicle-treated WT, vehicle-treated SCA3, and ASO-5-treated SCA3 mice at 16 weeks of age. Panels (A) through (G) are from recordings in the anterior cerebellar lobules, while panels (H) through (N) are from recordings in the nodular zone. In the anterior cerebellum, (A) firing frequency is not altered among all groups, nor is (B) spike regularity, as denoted by coefficient of variation (CV) of the interspike interval (ISI). (C) Action potential threshold was not altered among all groups. (D) Representative overlays of individual spikes from WT Veh, SCA3 Veh, and SCA3 ASO-5 Purkinje neurons in the anterior cerebellum. (E) Shape and characteristics of the afterhyperpolarization (AHP) and ISI were not significantly altered between WT Veh and SCA3 Veh, while SCA3 ASO-5 resulted in hyperpolarization throughout the ISI. (F) Spike half-width, (G) maximum slope of depolarization, (H) maximum slope of repolarization, and (I) steady state membrane potential were not altered among all groups. In the posterior cerebellum, (J) firing frequency is not altered among all groups, nor is (K) spike regularity as denoted by CV of the ISI. (L) Action potential threshold was not altered among all groups. (M) Representative overlays of individual spikes from WT Veh, SCA3 Veh, and SCA3 ASO-5 Purkinje neurons in the nodular zone. (N) Characteristics of the AHP and ISI were not altered among all groups. (O) Spike half-width was increased in SCA3 ASO-5 compared to SCA3 Veh, but this effect was modest and not significantly different from WT Veh. (P) Maximum slope of depolarization, (Q) maximum slope of repolarization, and (R) steady state membrane potential were not altered among all groups. * = p<0.05, *** = p<0.001, one-way ANOVA with Holm-Sidak correction for multiple comparisons, except in (E) and (N), using two-way repeated measures ANOVA with Holm-Sidak correction for multiple comparisons.