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Validation of Neuropilin-1 targeting by miR-24. Luciferase activity was measured in hBMECs 48 h after transfection, using the vector without Neuropilin-1 3′-UTR (empty vector), the vector containing the wild-type Neuropilin-1 3′-UTR, and the vector containing a mutated Neuropilin-1 3′-UTR (Neuropilin-1 MUT); a non-targeting miRNA (miR scramble) has been employed as further control. Means ± S.E.M; * p < 0.05.
