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. 2021 Feb 15;10(1):8. doi: 10.3390/antib10010008

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Dynamic Light Scattering (DLS) results for various constructs and HC-104 variants. (A) Tabulated molecular weight (M_W), known from sequence, and interaction parameter (kD) and hydrodynamic radius (R_h) obtained by DLS for both W104 and F104 variants in the Fab, Fab’2, and mAb forms. Highly negative kDs and large R_h values are in red font. (B) kD tabulated for Fc and Fab constructs for W104 and F104, and Fab/Fc mixtures (1:1 molar ratio). (C) Calculated M_w values derived from measured diffusional coefficients plotted against concentration for Fab, Fab’2, and mAb constructs for F104 (orange symbols) and W104 (blue symbols). Fab, Fab’2, and mAb are designated as diamonds, squares, and open circles, respectively. (D) Calculated M_w values derived from measured diffusional coefficients for Fab forms only of W104 (blue diamonds) and F104 (orange squares).