Figure 7.

(A) kD (mL/g) obtained by DLS (1–10 mg/mL in PBS) shown as bar plots for all variants engineered at HC-104, LC-Y30, and LC-F92. Obtained kD values less than −30 mL/g are shaded red bars, and those higher than −20 mL/g are shaded green. Values between −20 and −30 mL/g are shaded black. Error bars are shown. (B) kD values obtained for all 19 interface mutants are plotted against corresponding ∆AC-SINS values (blue circles with error bars for each method and the R² of the parabolic fit). Each data point is labeled by variant. (C) kD values obtained for all 19 interface mutants are plotted against corresponding R_h values (nm) obtained at both 1 mg/mL (orange circles) and 10 mg/mL (blue circles) along with R² of the linear fits.